ARGS-aggrecan quantification in synovial fluid, serum, plasma and urine - an assay validation
نویسندگان
چکیده
منابع مشابه
Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis
INTRODUCTION Aggrecanase cleavage at the (392)Glu-(393)Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal (393)ARGS fragments, is an early key event in arthritis and joint injuries. We determined whether synovial fluid (SF) levels of ARGS-aggrecan distinguish subjects with progressive radiographic knee osteoarthritis (ROA) from those with stable or no ROA. METHODS We...
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Putrescine (1,4-diaminobutane) and the polyamines spermidine and spermine have attracted interest as possible markers of malignancy and of response to chemotherapy. The assay methods currently in use for these amines are reviewed and compared in respect to sensitivity, reproducibility, and applicability to routine analysis for them in urine, blood, and cerebrospinal fluid.
متن کاملSynovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: a cross-sectional study
INTRODUCTION Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human synovial fluid to determine if this cleavage-site specific method better identifies joint pathology than pre...
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Synovial fluid is generally considered to be a dialysate of plasma to which mucin has been added during its passage through the synovial membrane (Ropes and Bauer, 1953). The proteins of the serum and synovial fluid have been shown to be qualitatively identical (Schmid and MacNair, 1956) and the concentrations of the various proteins in synovial fluid probably depend upon the following factors:...
متن کاملHydroxypyridinium collagen crosslinks in serum, urine, synovial fluid and synovial tissue in patients with rheumatoid arthritis compared with osteoarthritis.
OBJECTIVE To investigate the relationship between inflammation markers and content of pyridinium crosslinks in hydrolysates of synovial tissue and to specify the significance of urinary excreted pyridinoline, released primarily from collagen I and II of bone and cartilage, and deoxypyridinoline released especially from collagen I of bone and dentin, dependent on disease activity in rheumatoid a...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2014
ISSN: 1063-4584
DOI: 10.1016/j.joca.2014.02.105